The image shows the DNA distribution in different nuclei contained within a pluripotent zebrafish embryo. The image data were obtained with a Stimulated Emission Depletion (STED) super-resolution microscope at the Karlsruhe Center for Optics and Photonics by Lennart Hilbert. The image was used as the June 2021 issue cover for PLoS Computational Biology

Illustrations of the stages “oblong”, “sphere”, “dome”, “30% epiboly” and “50% epiboly” of the embryonic development in zebrafish. At the reference temperature of 28.5°C, the developmental stages occur at the indicated time points, measured in hours post fertilization (hpf). Illustration by Xenia Tschurikow.

The figure shows actin bundle formation after mitosis. Actin was visualized using recombinantly expressed actin chromobody with green fluorescent protein as a fluorescence marker and a nuclear localization sequence (NLS) to restrict detection to within the cell nucleus (nAC-GFP, generous gift from Grosse Lab, Uni Freiburg). Time-lapse images are single confocal sections chosen from z-stacks […]